Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Packaging

1 L in poly bottle

100, 500 mL in poly bottle

5 gal in poly drum

1 gal in poly bottle

5 mL in glass bottle

Application

Triton^™-X has been used:
• In the permeabilization of cells for immunofluorescence staining^[1]
• As a component of lysis buffer in western blot analysis^[1]
• As a component of Tris-buffered saline for the preparation of cell sections in Immunogold labelling for electron microscope^[1]

General description

Triton^™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.^[3][4]

Legal Information

Tri-Chek is a trademark of Micro Essential Laboratory, Inc.

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow